Article
Expression of the yeast plasma membrane [H+]ATPase in secretory vesicles. A new strategy for directed mutagenesis.
The Journal of biological chemistry - 25 Apr 1991
Nakamoto R K, Rao R, Slayman C W
Abstract excerpt
Secretory vesicles that accumulate in the temperature-sensitive sec6-4 strain of yeast have been shown to contain a vanadate-sensitive ATPase, presumably en route to the plasma membrane (Walworth, N. C., and Novick, P. J. (1987) J. Cell Biol. 105, 163-174). We have now established this enzyme to...
Topics
- Catalysis
- Chromatography, Gel
- Cytoplasmic Granules
- Electrophoresis, Polyacrylamide Gel
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Fungal
- Hydrogen-Ion Concentration
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Proton-Translocating ATPases
- Saccharomyces cerevisiae
- Vanadates