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Article

Biochemical characterization of laboratory mutants of extended-spectrum beta-lactamase TEM-60.

Antimicrobial agents and chemotherapy - 1 Sept 2004

Caporale Bibiana, Franceschini Nicola, Perilli Mariagrazia, Segatore Bernardetta, Rossolini Gian Maria, Amicosante Gianfranco

Abstract excerpt

Three mutants of the extended-spectrum beta-lactamase TEM-60, the P51L, K104E, and S164R mutants, were constructed by site-directed mutagenesis. The kinetic parameters of the mutated enzymes and interactions of inhibitors were significantly different from those of TEM-60, revealing that the L51P mutation plays an important role in enzyme activity and stability in the TEM-60 background.

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Identifiers

PMID: 15328132
DOI: 10.1128/AAC.48.9.3579-3582.2004
PMCID: PMC514782

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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