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Article

Analysis of the importance of the metallo-beta-lactamase active site loop in substrate binding and catalysis.

Chemistry & biology - 1 Apr 2003

Moali Catherine, Anne Christine, Lamotte-Brasseur Josette, Groslambert Sylvie, Devreese Bart, Van Beeumen Jozef, Galleni Moreno, Frère Jean Marie

Abstract excerpt

The role of the mobile loop comprising residues 60-66 in metallo-beta-lactamases has been studied by site-directed mutagenesis, determination of kinetic parameters for six substrates and two inhibitors, pre-steady-state characterization of the interaction with chromogenic nitrocefin, and molecular modeling. The W64A mutation was performed in IMP-1 and BcII (after replacement of the BcII 60-66 peptide by that of...

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PMID: 12725860
DOI: 10.1016/s1074-5521(03)00070-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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