Article
Light-stable rhodopsin. II. An opsin mutant (TRP-265----Phe) and a retinal analog with a nonisomerizable 11-cis configuration form a photostable chromophore.
The Journal of biological chemistry - 5 Apr 1992
Ridge K D, Bhattacharya S, Nakayama T A, Khorana H G
Abstract excerpt
In order to prepare a completely light-stable rhodopsin, we have synthesized an analog, II, of 11-cis retinal in which isomerization at the C11-C12 cis-double bond is blocked by formation of a cyclohexene ring from the C10 to C13-methyl. We used this analog to generate a rhodopsin-like pigment fr...
Topics
- Amino Acid Sequence
- Animals
- Blotting, Western
- Cattle
- Electrophoresis, Polyacrylamide Gel
- Eye Proteins
- G-Protein-Coupled Receptor Kinase 1
- Isomerism
- Light
- Molecular Sequence Data
- Mutation
- Phosphorylation
- Photochemistry
- Protein Kinases
- Rhodopsin
- Rod Opsins
- Spectrum Analysis