Article
In situ hybridization analysis of RGS mRNA regulation and behavioral phenotyping of RGS mutant mice.
Methods in enzymology - 1 Jan 2004
Gold Stephen J, Zachariou Venetia
Abstract excerpt
To elucidate the functional role of regulators of G-protein signaling (RGS) in vivo, it will be critical to (i) determine how RGS activity is altered in response to a variety of manipulations and (ii) observe how the system is changed when RGS protein function is altered genetically. To facilitate studies of dynamic regulation of RGS protein activity, this article describes detailed methods for radioisotopic in...
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