Article
A method of high frequency virus-induced gene silencing in chili pepper (Capsicum annuum L. cv. Bukang).
Molecules and cells - 30 Apr 2004
Chung Eunsook, Seong Eunsoo, Kim Yeoung-Cheol, Chung Eun Joo, Oh Sang-Keun, Lee Sanghyeob, Park Jeong Mee, Joung Young Hee, Choi Doil
Abstract excerpt
Using a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system, expression of phytogene desaturase (PDS) and ribulose-1,5-bisphosphate carboxylase small-subuit (rbcS) genes was suppressed in Nicotiana benthamiana and pepper plants (Capsicum annuum L. cv. Bukang). The silenced phenotypes of pale yellow (rbcS), and photobleached leaves (PDS), were invariably obvious 2 weeks after inoculation...
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