Stable inheritance of shuttle vectors based on plasmid pIM13 in a mutant strain of Clostridium acetobutylicum.

doi:10.1099/00221287-138-7-1371

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Article

Stable inheritance of shuttle vectors based on plasmid pIM13 in a mutant strain of Clostridium acetobutylicum.

Journal of general microbiology - 1 Jul 1992

Azeddoug H, Hubert J, Reysset G

Abstract excerpt

New shuttle vectors for Clostridium acetobutylicum were constructed, using as replicons the Gram-positive plasmid pIM13, and derivatives of the Gram-negative plasmid pBR322, including pUC19. These vectors transformed C. acetobutylicum at a high frequency (up to 10(6) transformants per microgram D...

Topics

Cloning, Molecular
Clostridium
Electrophoresis, Agar Gel
Genetic Vectors
Mutation
Plasmids

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