Article
A vector for controlled, high-yield production of specifically mutated proteins in Escherichia coli: test of a putative cytidine-binding domain in Rho factor and its Thr16----Ala mutant.
Gene - 1 Sept 1992
Richardson L V, Richardson J P
Abstract excerpt
A derivative of the plasmid vector, pET-3a [Rosenberg et al., Gene 56 (1987) 125-135], is described that contains the origin of replication from bacteriophage f1. This plasmid is well-suited for oligodeoxyribonucleotide mutagenesis and controlled production of mutant proteins from a single vector...
Topics
- Base Sequence
- Binding Sites
- Cytidine
- Escherichia coli
- Genetic Vectors
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oligoribonucleotides
- Peptide Chain Termination, Translational
- Recombinant Proteins
- Rho Factor
- Transcription, Genetic