Article
Simple Algorithm for Identification of Bordetella pertussis Pertactin Gene Variants.
Journal of clinical microbiology - 1 Apr 2004
Muyldermans Gaëtan, Piérard Denis, Hoebrekx Nathalie, Advani Reza, Van Amersfoorth Shirley, De Schutter Iris, Soetens Oriane, Eeckhout Leo, Malfroot Anne, Lauwers Sabine
Abstract excerpt
Studies performed in several countries have demonstrated the recent emergence and subsequent dominance of circulating Bordetella pertussis strains harboring pertactin and pertussis toxin variants not included in pertussis vaccines. Determination of the pertactin gene variants is commonly performed using a time-consuming and expensive sequence analysis. We developed a simple and reliable pertactin typing algorithm...
Topics
- Algorithms
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Bacterial Typing Techniques
- Base Sequence
- Bordetella pertussis
- Genetic Variation
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Sequence Analysis, DNA