Article
Improvement of BF typing and of BF F subtyping after neuraminidase treatment in the unconverted and converted factor B.
Electrophoresis - 1 Jun 1992
Siemens I, Geserick G, Mauff G, Correns A, Schröder H
Abstract excerpt
When neuraminidase-treated sera are analyzed by agarose gel isoelectric focusing, the factor B (BF) banding pattern is reduced to predominantly one major band without cathodically positioned bands. This not only makes unequivocal typing of BF allotypes possible but also the reliable distinction of all BF F subtype phenotypes with delimitation of "BF F subtype variants". With this new method, serum aging affects...
Topics
- Blood Preservation
- Complement Factor B
- Humans
- Hydrogen-Ion Concentration
- Immunoblotting
- Isoelectric Focusing
- Neuraminidase
- Phenotype
- Polymorphism, Genetic