Article
Hereditary subtypic patterns detected in the Ba fragment of complement factor B: occurrence of four common alleles in Japanese.
Electrophoresis - 1 Oct 1990
Suzuki K, Ito S, Tamura A, Fujita K, Matsumoto H
Abstract excerpt
A procedure which can detect subtype-specific minor bands of factor B (BF) by polyacrylamide gel isoelectric focusing is presented. After zymosan-mediated fragmentation of BF in serum via alternative pathway for complement activation, serum samples are subjected to isoelectric focusing in a narrow pH range (4.2-4.9). The Ba fragments are detected by using immunoblotting. In addition to the previously reported...
Topics
- Alleles
- Complement Activation
- Complement Factor B
- Gene Frequency
- Humans
- Hydrogen-Ion Concentration
- Isoelectric Focusing
- Japan
- Neuraminidase
- Peptide Fragments
- Phenotype