Article
Purification and characterization of the diphtheria toxin repressor.
Proceedings of the National Academy of Sciences of the United States of America - 15 Aug 1992
Schmitt M P, Twiddy E M, Holmes R K
Abstract excerpt
The diphtheria toxin repressor gene (dtxR) encodes a protein (DtxR) that regulates transcription of the diphtheria toxin gene (tox) by an iron-dependent mechanism. Cloned dtxR was expressed in Escherichia coli from the phage T7 gene 10 promoter, and DtxR was purified. Specific binding of DtxR to...
Topics
- Alleles
- Bacterial Proteins
- Base Sequence
- Chromatography, DEAE-Cellulose
- Cloning, Molecular
- Corynebacterium diphtheriae
- DNA, Bacterial
- DNA-Binding Proteins
- Diphtheria Toxin
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genes, Regulator
- Molecular Sequence Data
- Oligodeoxyribonucleotides
- Operon
- Oxidation-Reduction
- Polymerase Chain Reaction