Article
Trafficking of syngeneic murine lymphokine activated killer T cells following intraperitoneal administration in normal and tumor bearing mice.
Gynecologic oncology - 1 Aug 1992
Cappuccini F, Lucci J A, Dett C A, Gatanaga M, Ininns E K, Gatanaga T, Yamamoto R S, Manetta A, DiSaia P J, Granger G A
Abstract excerpt
Nongenetically restricted T cells may be important host effector cells in women with ovarian cancer receiving intraperitoneal (ip) IL-2 therapy. We developed an in vitro technique to produce murine lymphokine-activated killer T cells. Murine splenocytes were cultured in the presence of 1000 U/ml IL-2 for 10 to 15 days. Phenotypical analysis showed 95% of total cells to express the pan T phenotype Thy 1.2 and no...
Topics
- Animals
- Cell Count
- Cell Division
- Chromium Radioisotopes
- Cytotoxicity, Immunologic
- Female
- Injections, Intraperitoneal
- Killer Cells, Lymphokine-Activated
- Mice
- Mice, Inbred C57BL