Article
Genotyping using single nucleotide polymorphism, fluorescence spectroscopy and pattern recognition.
The Analyst - 1 Mar 2004
Brereton Richard G, Devonshire Martin
Abstract excerpt
This paper describes a method for genetic screening using single nucleotide polymorphism. Fluorescence spectra with an excitation frequency of 488 nm are recorded over a range of 550 to 660 nm of fragments of human DNA together with two fluorescent probe dyes attached to specific primers, one for each type of allele and a background dye, prepared using the Taqman reaction. The fluorescence spectra are monitored...
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