Article
Intracellular phosphate serves as a signal for the regulation of the PHO pathway in Saccharomyces cerevisiae.
The Journal of biological chemistry - 23 Apr 2004
Auesukaree Choowong, Homma Tomoyuki, Tochio Hidehito, Shirakawa Masahiro, Kaneko Yoshinobu, Harashima Satoshi
Abstract excerpt
In Saccharomyces cerevisiae, the phosphate signal transduction pathway (PHO pathway) is known to regulate the expression of several phosphate-responsive genes, such as PHO5 and PHO84. However, the fundamental issue of whether cells sense intracellular or extracellular phosphate remains unresolved. To address this issue, we have directly measured intracellular phosphate concentrations by (31)P NMR spectroscopy. We...
Topics
- Biological Transport
- Dose-Response Relationship, Drug
- Gene Expression Regulation, Fungal
- Genotype
- Magnetic Resonance Spectroscopy
- Models, Genetic
- Phenotype
- Phosphates
- Proton-Phosphate Symporters
- Saccharomyces cerevisiae