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Article

Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.

Emerging infectious diseases - 1 Jan 2000

Pedraza-Díaz S, Amar C, Nichols G L, McLauchlin J

Abstract excerpt

We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven...

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Identifiers

PMID: 11266294
DOI: 10.3201/eid0701.700049
PMCID: PMC2631669

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