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Detection of Cryptosporidium spp. from human faeces by PCR-RFLP, cloning and sequencing.

Parasitology research - 1 Feb 2009

Leone Annalisa, Ripabelli Giancarlo, Sammarco Michela Lucia, Grasso Guido Maria

Abstract excerpt

In this study, we compared and validated a nested ABC polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay to amplify Cryptosporidium parvum oocyst wall protein (COWP) gene fragment with a previous nested PCR-RFLP method. When RFLP of COWP gene did not provide clear results, we further analysed samples by cloning and sequencing for species and genotype identification. ABC-PCR was...

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PMID: 18979120
DOI: 10.1007/s00436-008-1233-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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