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Detection of <i>Cryptosporidium</i> and Identification to the Species Level by Nested PCR and Restriction Fragment Length Polymorphism

1 Mar 2005

Abstract excerpt

Cryptosporidiosis is an emerging protozoan disease associated with large waterborne outbreaks. Diagnosis relies on microscopic examination of stools, but this method cannot identify the infecting species of Cryptosporidium. We have developed a test based on nested PCR and restriction fragment length polymorphism (RFLP) that offers simple identification of Cryptosporidium hominis, Cryptosporidium parvum, and most...

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PMID: 15750054
DOI: 10.1128/jcm.43.3.1017-1023.2005

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