Article
Downstream activating sequence within the coding region of a yeast gene: specific binding in vitro of RAP1 protein.
Molecular & general genetics : MGG - 1 Dec 1992
Fantino E, Marguet D, Lauquin G J
Abstract excerpt
Using a gel retardation assay, a protein factor that specifically interacts with a 33 bp intragenic sequence of the highly expressed and glucose-inducible SRP1 gene of Saccharomyces cerevisiae has been detected. This binding site is located in a transcribed region and within the open reading frame (positions +710 to +743 relative to the first base of the initiation codon). A mutant strain carrying a deletion of...
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