Participation of ABF-1 protein in expression of the Saccharomyces cerevisiae CAR1 gene.

Abstract excerpt

DNA fragments previously shown to be required for expression of the CAR1 (arginase) gene in Saccharomyces cerevisiae and to support transcriptional activation of a reporter gene in a heterologous expression vector were shown to bind purified regulatory protein ABF-1. Two ABF-1 sites were identified in the CAR1 upstream region, one to which ABF-1 protein bound with high affinity and a second to which it bound much...

Topics

• Arginase
• Base Sequence
• Binding Sites
• Cloning, Molecular
• DNA-Binding Proteins
• Fungal Proteins
• Gene Expression Regulation, Fungal
• Molecular Sequence Data
• Mutation
• Oligonucleotides
• Regulatory Sequences, Nucleic Acid