Article
Rapid and automated characterisation of seed genotype using Micrograd electrophoresis and pattern-matching software.
Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society - 1 Jan 1992
Wrigley C W, Batey I L, Bekes F, Gore P J, Margolis J
Abstract excerpt
New precast microgels are described for use in quickly identifying seed of cereal varieties by determining protein composition within an hour. For example, gliadin proteins are extracted from crushed wheat grain, wheatmeal or flour with ethylene glycol (centrifugation not necessary) and 5 microliters extract is applied to a Micrograd gel (3-15% gel gradient) for ten minutes' electrophoresis at 300 volts in sodium...
Topics
- Automation
- Edible Grain
- Electrophoresis, Polyacrylamide Gel
- Genotype
- Gliadin
- Pattern Recognition, Automated
- Seeds
- Software