Article
Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequences.
Nucleic acids research - 1 Jan 2004
McKinney Jeffrey S, Zhang Haifeng, Kubori Tomoko, Galán Jorge E, Altman Sidney
Abstract excerpt
The type III secretion system involved in Salmonella enterica serovar Typhimurium invasion of host cells has been disrupted using inducibly expressed oligonucleotide external guide sequences (EGSs) complementary to invB or invC mRNA. These EGSs direct single site cleavage in these mRNAs by endogenous RNase P, and their expression in Salmonella results in invC mRNA and InvC protein depletion, decreased type III...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.