Article
High-level multiplex genotyping of polymorphisms involved in folate or homocysteine metabolism by matrix-assisted laser desorption/ionization mass spectrometry.
Clinical chemistry - 1 Feb 2004
Meyer Klaus, Fredriksen Ase, Ueland Per Magne
Abstract excerpt
BACKGROUND: Increased plasma total homocysteine (tHcy), a risk factor for cardiovascular disease, is related to genetic, environmental, and nutritional factors, in particular folate status. Future large epidemiologic studies of the genetic basis of hyperhomocysteinemia will require high-throughput assays for polymorphisms of genes related to folate and Hcy metabolism. METHOD: We developed a high-level multiplex...
Topics
- Folic Acid
- Genotype
- Homocysteine
- Humans
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Single Nucleotide
- Reproducibility of Results
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization