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Article

Cell-based assay for the determination of temperature sensitive and cold adapted phenotypes of influenza viruses.

Journal of virological methods - 1 Mar 2004

Kiseleva I, Su Q, Toner T J, Szymkowiak C, Kwan W-S, Rudenko L, Shaw A R, Youil R

Abstract excerpt

The determination of temperature sensitive (ts) and cold adapted (ca) phenotype for influenza A and B strains has been conducted traditionally using embryonated chicken eggs. As attempts are made to move away from the use of eggs in the manufacturing process of influenza vaccines, it will become useful to develop cell-based assays to support cell culture-based vaccine production. In this study, MDCK cells have...

Topics

Animals
Cell Line
Chick Embryo
Cold Temperature
Dogs
Influenza A virus
Influenza B virus
Influenza Vaccines
Phenotype
Reassortant Viruses
Temperature

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Vaccines, Attenuated

Virus Replication

Source

Identifiers

PMID: 14715309
DOI: 10.1016/j.jviromet.2003.10.012

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