Article
Molecular definition of predictive indicators of stable protein expression in recombinant NS0 myeloma cells.
Biotechnology and bioengineering - 20 Jan 2004
Barnes Louise M, Bentley Catherine M, Dickson Alan J
Abstract excerpt
We have generated a molecular description of the loci at which stability/instability of expression of a monoclonal antibody (MAb) (anti-CD38) occurs within the GS-NS0 expression system. Critically, these data show that, in the absence of changes to copy number for the recombinant gene sequences, all cell lines examined exhibit a progressive loss (instability) in expression of mRNA during prolonged culture....
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