Article
Residue Asp-189 controls both substrate binding and the monovalent cation specificity of thrombin.
The Journal of biological chemistry - 12 Mar 2004
Prasad Swati, Cantwell Angelene M, Bush Leslie A, Shih Peter, Xu Hong, Di Cera Enrico
Abstract excerpt
Residue Asp-189 plays an important dual role in thrombin: it defines the primary specificity for Arg side chains and participates indirectly in the coordination of Na(+). The former role is shared by other proteases with trypsin-like specificity, whereas the latter is unique to Na(+)-activated proteases in blood coagulation and the complement system. Replacement of Asp-189 with Ala, Asn, Glu, and Ser drastically...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.