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[PCR-based site-directed mutagenesis and recombinant expression plasmid construction of a SCN5A mutation (K317N) identified in a Chinese family with Brugada syndrome].

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA - 1 Nov 2003

Yi Shao-dong, Meng Su-rong, Cui Ying-kai, Chen Zhe-ming, Peng Jian

Abstract excerpt

OBJECTIVE: To perform PCR-based site-directed mutagenesis of a new SCN5A mutation (K317N) identified in a Chinese family with Brugada syndrome and construct the recombinant expression plasmid pRc/CMV-Hh1 containing the human cardiac sodium channel alpha subunit (hH1), mutant cDNA. METHODS: A pair of primers was designed according to the restricted sites Sse 8387I and Age I of the SCN5A sequence with the...

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PMID: 14625171

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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