Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Real-time PCR with molecular beacons provides a highly accurate assay for detection of Tay-Sachs alleles in single cells.

Prenatal diagnosis - 1 Dec 2002

Rice John E, Sanchez J Aquiles, Pierce Kenneth E, Wangh Lawrence J

Abstract excerpt

The results presented here provide the first single-cell genetic assay for Tay-Sachs disease based on real-time PCR. Individual lymphoblasts were lysed with an optimized lysis buffer and assayed using one pair of primers that amplifies both the wild type and 1278 + TATC Tay-Sachs alleles. The resulting amplicons were detected in real time with two molecular beacons each with a different colored fluorochrome. The...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biological Assay
Share to DNA Probes
Share to Lymphocytes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Pregnancy

Share to Preimplantation Diagnosis

Share to Reproducibility of Results

Share to Reverse Transcriptase Polymerase Chain Reaction

Share to Tay-Sachs Disease

Source

Identifiers

PMID: 12454972
DOI: 10.1002/pd.500

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Real-time PCR with molecular beacons provides a highly accurate assay for detection of Tay-Sachs alleles in single cells.PMID 12454972Read ↗

Multiplexed fluorescence analysis for mutations causing Tay-Sachs disease.Semantic proximity Read ↗

The molecular basis of Tay-Sachs disease: mutation identification and diagnosis.Semantic proximity Read ↗

Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR.Semantic proximity Read ↗

Simultaneous amplification of the two most frequent mutations of infantile Tay-Sachs disease in single blastomeres.Semantic proximity Read ↗

Linear-after-the-exponential polymerase chain reaction and allied technologies. Real-time detection strategies for rapid, reliable diagnosis from single cells.Semantic proximity Read ↗

Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis.Semantic proximity Read ↗

Single-tube genotyping without oligonucleotide probes.Semantic proximity Read ↗

SNP genotyping using single-tube fluorescent bidirectional PCR.Semantic proximity Read ↗

Rapid single tube genotyping of ACP1 by FRET based amplification and dual color melting curve analysis.Semantic proximity Read ↗

Rapid detection of fetal Mendelian disorders: Tay-Sachs disease.Semantic proximity Read ↗

Tay-Sachs disease screening and diagnosis: evolving technologies.Semantic proximity Read ↗

Biallelic discrimination assays for the three common Ashkenazi Jewish mutations and a common non-Jewish mutation, in Tay-Sachs disease, using fluorogenic TaqMan probes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.