Article
A new approach to extending the wheat marker pool by anchored PCR amplification of compound SSRs.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Feb 2004
Hayden M J, Stephenson P, Logojan A M, Khatkar D, Rogers C, Koebner R M D, Snape J W, Sharp P J
Abstract excerpt
A study was undertaken to determine the utility in bread wheat of anchored PCR for the development of single locus SSR markers targeted at compound repeat motifs. In anchored PCR, microsatellite amplification is achieved using a single primer complementary to the flanking sequence, and one which anchors to the repeat junction of the compound SSR. The recovery rate of useable markers was found to be similar (43%)...
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