Article
Simple sequence repeat primers used in polymerase chain reaction amplifications to study genetic diversity in barley.
Genome - 1 Feb 1996
Sánchez de la Hoz M P, Dávila J A, Loarce Y, Ferrer E
Abstract excerpt
In combination with oligonucleotides of arbitrary sequence, 5' anchored oligonucleotides based on simple sequence repeats were used in polymerase chain reaction amplifications to produce barley DNA fingerprints. The aim of this work was (i) to develop a simple nonradioactive experimental procedure to reveal polymorphism in regions containing SSRs, (ii) to determine the genetic nature of polymorphisms, and (iii)...
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