Article
Robust allele-specific polymerase chain reaction markers developed for single nucleotide polymorphisms in expressed barley sequences.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Jan 2006
Bundock P C, Cross M J, Shapter F M, Henry R J
Abstract excerpt
Many methods have been developed to assay for single nucleotide polymorphisms (SNPs), but generally these depend on access to specialised equipment. Allele-specific polymerase chain reaction (AS-PCR) is a method that does not require specialised equipment (other than a thermocycler), but there is a common perception that AS-PCR markers can be unreliable. We have utilised a three primer AS-PCR method comprising of...
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