Article
Conversion of AFLP bands into high-throughput DNA markers.
Molecular genetics and genomics : MGG - 1 Apr 2001
Meksem K, Ruben E, Hyten D, Triwitayakorn K, Lightfoot D A
Abstract excerpt
The conversion of AFLP bands into polymorphic sequence-tagged-site (STS) markers is necessary for high-throughput genotype scoring. Technical hurdles that must be overcome arise from genome complexity (particularly sequence duplication), from the low-molecular-weight nature of the AFLP bands and from the location of the polymorphism within the AFLP band. We generated six STS markers from ten AFLP bands (four...
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