Article
Allelic polymorphism in the ovine DQA1 gene.
Journal of animal science - 1 Jan 2004
Zhou H, Hickford J G H
Abstract excerpt
Variation in the ovine DQA1 gene was investigated by amplification of exon 2 using PCR, followed by single-strand conformational polymorphism (SSCP) analysis, cloning, and DNA sequencing. Fourteen novel SSCP patterns, representing 14 different sequences, were identified. Eight of these 14 sequences were identical to published DQA1 sequences from sheep, whereas the remaining six were novel but similar to the...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- Cattle
- Exons
- Gene Amplification
- Genes, MHC Class II
- Genetic Variation
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Single-Stranded Conformational
- Sequence Alignment
- Sheep
- Species Specificity
