Article
Iron-regulated phenylalanyl-tRNA synthetase activity in Azotobacter vinelandii.
FEMS microbiology letters - 21 Jan 2003
Page William J, Mehrotra Manisha, Vande Woestyne Marleen, Tindale Anne E, Kujat Choy Sonya L, Macyk Andrea S, Leskiw Brenda K
Abstract excerpt
Azotobacter vinelandii strain UA22 was produced by pTn5luxAB mutagenesis, such that the promoterless luxAB genes were transcribed in an iron-repressible manner. Tn5luxAB was localized to a fragment of chromosomal DNA encoding the thrS, infC, rpmI, rplT, pheS and pheT genes, with Tn5 inserted in the 3'-end of pheS. The isolation of this mutation in an essential gene was possible because of polyploidy in...
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