Article
PRT1 of Arabidopsis is a ubiquitin protein ligase of the plant N-end rule pathway with specificity for aromatic amino-terminal residues.
Plant physiology - 1 Nov 2003
Stary Susanne, Yin Xiao-jun, Potuschak Thomas, Schlögelhofer Peter, Nizhynska Victoria, Bachmair Andreas
Abstract excerpt
The gene PRT1 of Arabidopsis, encoding a 45-kD protein with two RING finger domains, is essential for the degradation of F-dihydrofolate reductase, a model substrate of the N-end rule pathway of protein degradation. We have determined the function of PRT1 by expression in yeast (Saccharomyces cerevisiae). PRT1 can act as a ubiquitin protein ligase in the heterologous host. The identified substrates of PRT1 have...
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