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Probing C-terminal interactions of the Pseudomonas stutzeri cyanide-degrading CynD protein.

Applied microbiology and biotechnology - 1 Apr 2015

Crum Mary Abou-Nader, Park Jason M, Mulelu Andani E, Sewell B Trevor, Benedik Michael J

Abstract excerpt

The cyanide dihydratases from Bacillus pumilus and Pseudomonas stutzeri share high amino acid sequence similarity throughout except for their highly divergent C-termini. However, deletion or exchange of the C-termini had different effects upon each enzyme. Here we extended previous studies and investigated how the C-terminus affects the activity and stability of three nitrilases, the cyanide dihydratases from B....

Topics

Alanine
Aminohydrolases
Bacillus
Enzyme Stability
Hydro-Lyases
Hydrolases
Mutation
Neurospora crassa
Protein Multimerization
Pseudomonas stutzeri

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PMID: 25549622
DOI: 10.1007/s00253-014-6335-x

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