Article
New TCR transgenic model for tracking allospecific CD4 T-cell activation and tolerance in vivo.
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons - 1 Oct 2003
Sandner Sigrid E, Salama Alan D, Houser Stuart L, Palmer Ed, Turka Laurence A, Sayegh Mohamed H
Abstract excerpt
We have developed an adoptive transfer model system to visualize the dynamics of alloantigen-specific CD4+ T-cell activation in vivo. Using TCR-transgenic (tg) mice reactive to I-Ab(m12), we studied the clonal expansion and differentiation of alloreactive T cells by tracking the fate of adoptively transferred TCR-tg CD4+ T cells in syngeneic mice transplanted with skin grafts expressing I-Ab(m12). Following...
Topics
- Adoptive Transfer
- Animals
- Antibodies, Monoclonal
- CD4-Positive T-Lymphocytes
- Cytokines
- Flow Cytometry
- Immunohistochemistry
- Isoantigens
- Kinetics
- Lymphocyte Activation
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL