Rapid molecular diagnosis of hemoglobin variants by RT-PCR of reticulocyte mRNA and direct sequencing.

doi:10.3109/03630269209005689

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Article

Rapid molecular diagnosis of hemoglobin variants by RT-PCR of reticulocyte mRNA and direct sequencing.

Hemoglobin - 1 Jan 1992

Liu T C, Yen J S, Shen J S, Chen Y H, Lee L S, Chen P H, Chang J G

Abstract excerpt

We have developed a rapid and simple approach for the molecular characterization of hemoglobin variants by a one-step reverse transcription-polymerase chain reaction of reticulocyte mRNA and direct sequencing of the product. This method can selectively amplify the alpha 1- or alpha 2-globin gene...

Topics

Base Sequence
Genetic Variation
Globins
Hematologic Tests
Hemoglobins
Humans
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger
Reticulocytes

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