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Article

Rapid identification of beta-globin structural mutations by sequencing the mRNA from peripheral blood reticulocytes.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas - 1 Oct 1993

Miranda S R, Gonçalves M S, Sonati M F, Saad S T, Costa F F

Abstract excerpt

A simple and rapid method for the molecular detection of beta-globin structural mutations is described using a reverse transcription-polymerase chain reaction of reticulocyte mRNA and direct sequencing of the product. The amplified segment (employing a sense primer 5'-ATTTGCTTCTGACACAACTGT-3', located at position + 1 with respect to the Cap site and an antisense primer 5'-TCCAGATGCTCAAGGCCCTTC-3', located at...

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PMID: 8312834

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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