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Article

Rapid identification of beta-globin structural mutations by sequencing the mRNA from peripheral blood reticulocytes.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas - 1 Oct 1993

Miranda S R, Gonçalves M S, Sonati M F, Saad S T, Costa F F

Abstract excerpt

A simple and rapid method for the molecular detection of beta-globin structural mutations is described using a reverse transcription-polymerase chain reaction of reticulocyte mRNA and direct sequencing of the product. The amplified segment (employing a sense primer 5'-ATTTGCTTCTGACACAACTGT-3', lo...

Topics

Base Sequence
DNA Mutational Analysis
Globins
Humans
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
RNA, Messenger
Reticulocytes
Transcription, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 8312834

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8312834-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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