Article
Mutations at the putative junction sites of the yeast VMA1 protein, the catalytic subunit of the vacuolar membrane H(+)-ATPase, inhibit its processing by protein splicing.
Biochemical and biophysical research communications - 15 Oct 1992
Hirata R, Anraku Y
Abstract excerpt
A single gene, VMA1, encodes the 69-kDa subunit of the vacuolar membrane H(+)-ATPase in the yeast Saccharomyces cerevisiae. We have proposed that the subunit is synthesized as a precursor of 120 kDa (1,071 amino acids) and then converted to the 69-kDa form by an unusual processing reaction, which...
Topics
- Alternative Splicing
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Fungal
- Genes, Fungal
- Intracellular Membranes
- Macromolecular Substances
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligonucleotide Probes
- Phenotype
- Proton-Translocating ATPases
- Saccharomyces cerevisiae
- Vacuoles