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Article

Determining the effect of inducible protein phosphorylation on the DNA-binding activity of transcription factors.

Analytical biochemistry - 1 Jun 1992

Papavassiliou A G, Bohmann K, Bohmann D

Abstract excerpt

Inducible phosphorylation or dephosphorylation of transcription factors is an important mechanism of signal-dependent gene regulation in eukaryotic cells. This paper describes a combination of techniques that can be used to study the effect of this covalent protein modification on the DNA-binding activity of transcription factors. The protein of interest is genetically tagged with oligohistidine to allow rapid...

Topics

Amino Acid Sequence
Base Sequence
Blotting, Southern
Blotting, Western
DNA
DNA-Binding Proteins
Gene Expression Regulation, Bacterial
HeLa Cells
Humans
Molecular Sequence Data
Mutagenesis, Site-Directed

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Phosphorylation

Proto-Oncogene Proteins c-jun

Transcription Factors

Transcription, Genetic

Source

Identifiers

PMID: 1416026
DOI: 10.1016/0003-2697(92)90317-z

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1416026-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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