Article
Cloning and characterization of a gene which determines osmotic stability in Saccharomyces cerevisiae.
Molecular and cellular biology - 1 Aug 1991
Stateva L I, Oliver S G, Trueman L J, Venkov P V
Abstract excerpt
The srb1-1 mutation of Saccharomyces cerevisiae is an ochre allele which renders the yeast dependent on an osmotic stabilizer for growth and gives the cells the ability to lyse on transfer to hypotonic conditions. A DNA fragment which complements both of these phenotypic effects has been cloned....
Topics
- Chromosomes, Fungal
- Cloning, Molecular
- Culture Media
- Escherichia coli
- Gene Library
- Genes, Fungal
- Genetic Complementation Test
- Genotype
- Mutation
- Nucleic Acid Hybridization
- Osmolar Concentration
- Phenotype
- Plasmids
- RNA, Messenger
- Restriction Mapping
- Saccharomyces cerevisiae
- Transcription, Genetic
- Transformation, Genetic