Role of N- and C-terminal amino acids in antithrombin binding to pentasaccharide.

Abstract excerpt

We have used a monoclonal antibody-based binding procedure to determine the dissociation constants of the interactions between the essential antithrombin-binding pentasaccharide and a series of 13 distinct N- and C-terminal antithrombin substitution mutation variants with defective binding interaction with heparin. The reduction in binding affinity of the pentasaccharide with the N-terminal variants (with...

Topics

• Amino Acids
• Antithrombins
• Binding Sites
• Heparin
• Humans
• Mutation
• Oligosaccharides