Article
Correction of the apical membrane chloride permeability defect in polarized cystic fibrosis airway epithelia following retroviral-mediated gene transfer.
Human gene therapy - 1 Jun 1992
Olsen J C, Johnson L G, Stutts M J, Sarkadi B, Yankaskas J R, Swanstrom R, Boucher R C
Abstract excerpt
We are studying the introduction and expression of the normal cystic fibrosis transmembrane conductance regulator (CFTR) cDNA into cultured human airway epithelial cells as a model for gene therapy of cystic fibrosis. In this paper, we show that the chloride transport defect at the apical membrane is corrected in vitro in differentiated ion-transporting CF airway epithelial cells that exhibit polarized properties...
Topics
- 3T3 Cells
- Amiloride
- Animals
- Base Sequence
- Bronchi
- Cell Differentiation
- Cell Line
- Cell Membrane Permeability
- Chloride Channels
- Chlorides
- Cloning, Molecular