Article
Enrichment and characterization of peripheral blood-derived megakaryocyte progenitors that mature in short-term liquid culture.
Stem cells (Dayton, Ohio) - 1 Sept 1994
Nichol J L, Hornkohl A C, Choi E S, Hokom M M, Ponting I, Schuening F W, Hunt P
Abstract excerpt
A population of peripheral blood-derived cells that mature into megakaryocytes within four to eight days of liquid culture is described. This population was enriched from normal leukapheresis units by counterflow centrifugal elutriation and CD34 selection. The cells were incubated in suspension with known megakaryocyte growth or maturation factors. Megakaryocytes were identified within the cultures with...
Topics
- Anemia, Aplastic
- Animals
- Antigens, CD
- Antigens, CD34
- Blood Cells
- Blood Donors
- Cell Differentiation
- Cell Separation
- Cells, Cultured
- Culture Media
- Culture Techniques