Article
Detection of both mutant and wild-type p53 protein in normal skin fibroblasts and demonstration of a shared 'second hit' on p53 in diverse tumors from a cancer-prone family with Li-Fraumeni syndrome.
Oncogene - 1 May 1992
Srivastava S, Tong Y A, Devadas K, Zou Z Q, Sykes V W, Chen Y, Blattner W A, Pirollo K, Chang E H
Abstract excerpt
Germline transmission of mutant p53 gene in cancer-prone families with Li-Fraumeni syndrome has revealed a new role for p53 in the genetic predisposition to cancer. The studies reported here focus on the analysis of the expression of normal and mutant p53 RNA and protein in germline configuration and demonstrate that normal skin fibroblasts derived from members of a family with Li-Fraumeni syndrome express mutant...
Topics
- Alleles
- Base Sequence
- Blotting, Northern
- Electrophoresis, Gel, Two-Dimensional
- Fibroblasts
- Gene Expression
- Humans
- Li-Fraumeni Syndrome
- Molecular Sequence Data
- Mutation