Article
Intramolecular relationships in cholinesterases revealed by oocyte expression of site-directed and natural variants of human BCHE.
The EMBO journal - 1 Apr 1992
Neville L F, Gnatt A, Loewenstein Y, Seidman S, Ehrlich G, Soreq H
Abstract excerpt
Structure-function relationships of cholinesterases (CHEs) were studied by expressing site-directed and naturally occurring mutants of human butyrylcholinesterase (BCHE) in microinjected Xenopus oocytes. Site-directed mutagenesis of the conserved electronegative Glu441,Ile442,Glu443 domain to Gly...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Butyrylcholinesterase
- Cholinesterase Inhibitors
- Cholinesterases
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Female
- Genetic Variation
- Humans
- Kinetics
- Models, Molecular
- Molecular Sequence Data