Amplification of the topoisomerase II alpha gene in a non-small cell lung cancer cell line and characterisation of polymorphisms at the human topoisomerase II alpha and beta loci in normal tissue.

Abstract excerpt

DNA was prepared from normal tissue and 19 lung cancer cell lines. Using probes which detect restriction fragment length polymorphisms at both the topoisomerase II alpha and beta loci, heterozygosity was detected at a frequency of 0.17 and 0.37 for the alpha and beta loci, respectively. Southern...

Topics

• Adenocarcinoma
• Carcinoma, Non-Small-Cell Lung
• DNA Topoisomerases, Type II
• Deoxyribonucleases, Type II Site-Specific
• Gene Amplification
• Genotype
• Humans
• Lung Neoplasms
• Polymorphism, Restriction Fragment Length
• Tumor Cells, Cultured