Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Modification of primers for GRHPR genotyping: avoiding allele dropout by single nucleotide polymorphisms and homology sequence.

Urological research - 1 Dec 2008

Takaoka Naohisa, Takayama Tatsuya, Miyazaki Miki, Nagata Masao, Ozono Seiichiro

Abstract excerpt

Mutation of primer site for genotyping by polymerase chain reaction (PCR) may cause allele dropout and other genotyping failures. Primary hyperoxaluria type 2 (PH2) is a rare inherited disease caused by overproduction of endogenous oxalate due to mutations in the glyoxylate/hydroxypyruvate reductase (GRHPR) gene. Here, to avoid allele dropout and primer annealing to multiple sites, and given the discrepancy in...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alcohol Oxidoreductases
Share to DNA

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Primers

Share to Hyperoxaluria, Primary

Share to Introns

Share to Linkage Disequilibrium

Share to Sequence Homology, Nucleic Acid

Source

Identifiers

PMID: 18982322
DOI: 10.1007/s00240-008-0159-z

Genes

GRHPR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Modification of primers for GRHPR genotyping: avoiding allele dropout by single nucleotide polymorphisms and homology sequence.PMID 18982322Read ↗

Molecular analysis of the glyoxylate reductase (GRHPR) gene and description of mutations underlying primary hyperoxaluria type 2.Semantic proximity Read ↗

Genetic basis of primary hyperoxaluria type II.Semantic proximity Read ↗

The gene encoding hydroxypyruvate reductase (GRHPR) is mutated in patients with primary hyperoxaluria type IISemantic proximity Read ↗

Identification of missense, nonsense, and deletion mutations in the GRHPR gene in patients with primary hyperoxaluria type II (PH2)Semantic proximity Read ↗

Late diagnosis of primary hyperoxaluria type 2 in the adult: effect of a novel mutation in GRHPR gene on enzymatic activity and molecular modeling.Semantic proximity Read ↗

Primary hyperoxaluria type 2 in children.Semantic proximity Read ↗

An overview of the role of genotyping in the diagnosis of the primary hyperoxalurias.Semantic proximity Read ↗

Ethnic differences in GRHPR mutations in patients with primary hyperoxaluria type 2.Semantic proximity Read ↗

Allele-dropout using PCR-based diagnosis for the splicing mutation in intron-2 of the CYP21B-gene: successful amplification with a Taq/Pwo-polymerase mixture.Semantic proximity Read ↗

Mutations in DHDPSL are responsible for primary hyperoxaluria type III.Semantic proximity Read ↗

Sequence specific generation of a DNA panhardle permits PCR amplication of unknown flanking DNASemantic proximity Read ↗

A new genotyping method for detecting low abundance single nucleotide mutations based on gap ligase chain reaction and quantitative PCR assay.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.