Article
Calcium requirement for protoplast transfection mediated by polyethylene glycol of Lactobacillus casei by PL-1 Phage DNA.
Bioscience, biotechnology, and biochemistry - 1 Nov 1992
Watanabe K, Kakita Y, Nakashima Y, Miake F
Abstract excerpt
The effects of some divalent cations on protoplast transfection mediated by polyethylene glycol of Lactobacillus casei ATCC 27092 by PL-1 phage DNA in 50 mM Tris-maleate buffer (pH 6.0) were investigated. The efficiency of transfection increased about 30 times in the presence of 10 mM Ca2+. Sr2+ increased the transfection rate as well, but Ba2+, Mn2+, and Mg2+ did not. Co2+ and Zn2+ inhibited transfection. The...
Topics
- Bacteriophages
- Biotechnology
- Calcium
- Cations, Divalent
- DNA, Viral
- Lacticaseibacillus casei
- Mutation
- Polyethylene Glycols
- Protoplasts
- Transfection